使用两步PCR和下一代16S rRNA基因测序进行微生物群分析
27,894 Views
•
11:22 min
•
October 15, 2019
DOI:
Summary
Automatically generated
这里描述的是使用16S rRNA基因组测序和利用免费工具进行分析的微生物群分析的简化标准操作程序。该协议将帮助那些对微生物群领域较新的研究人员，以及那些需要更新方法以更高分辨率实现细菌分析的研究人员。
Explore More Videos
Related Videos
09:55
Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
Related Videos
26793 Views
07:42
Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
Related Videos
4123 Views
06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Related Videos
17922 Views
09:09
A Method for Targeted 16S Sequencing of Human Milk Samples
Related Videos
9826 Views
08:33
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
Related Videos
12955 Views
05:28
Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method
Related Videos
2125 Views
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Related Videos
83397 Views
12:37
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Related Videos
38551 Views
08:05
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Related Videos
19635 Views
07:21
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Related Videos
12829 Views
Read Article
Cite this Article
Shahi, S. K., Zarei, K., Guseva, N. V., Mangalam, A. K. Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing. J. Vis. Exp. (152), e59980, doi:10.3791/59980 (2019).
Copy