Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

Caitlin  E Tolbert; Lindsey Palmquist; Hannah  Lee Dixon; Melissa  C. Srougi

doi:10.3791/59989

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress

在氧化应激期间细胞粘附和纤维蛋白扩散动力学

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10:57 min

October 13, 2019

DOI: 10.3791/59989-v

Caitlin E Tolbert¹, Lindsey Palmquist², Hannah Lee Dixon², Melissa C. Srougi^2,3

¹Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, ²Department of Chemistry,High Point University, ³Biotechnology Program and the Department of Molecular Biomedical Sciences,North Carolina State University

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该方法可用于量化细胞粘附的早期动力学，并将锚固依赖细胞扩散到纤维质。此外，此测定可用于研究改变的氧化还原平衡对细胞扩散和/或细胞粘附相关细胞内信号通路的影响。

这种方法将使研究人员能够量化细胞粘附的早期动力学，并在氧化应激期间将锚状依赖细胞扩散到细胞外基质蛋白纤维素上。该技术用途广泛，可适应于在多种条件下检查细胞骨骼动力学。此外，数据采集和分析使用通用实验室设备和免费软件进行。

了解细胞在改性氧化还原状态期间如何连接和扩散 ECM 的机制，可以提供有关正常和疾病状态（如转移性癌症）的宝贵见解。此方法可用于检查不同细胞培养条件下的细胞扩散和粘附、锚系依赖细胞系和/氧化剂，以回答广泛的生物学问题。执行此技术需要大量试剂。

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生物化学问题 152 细胞粘附细胞扩散氧化应激纤维素上皮细胞 ATM抑制褶皱应激纤维细胞循环