Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Geetika Bajpai; Kory  J. Lavine

doi:10.3791/60015

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

从人类和小鼠心肌标本中分离巨噬细胞子集和细胞位细胞

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07:25 min

December 17, 2019

DOI: 10.3791/60015-v

Geetika Bajpai¹, Kory J. Lavine^1,2,3

¹Department of Medicine,Washington University School of Medicine, ²Department of Immunology and Pathology,Washington University School of Medicine, ³Department of Developmental Biology,Washington University School of Medicine

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这里介绍的一个协议，通过酶消化制备单个细胞悬浮液，从人类和小鼠心肌中分离出巨噬细胞和其他非免疫细胞的各种子集。提出了基于流细胞学的识别与分离巨噬细胞表征的门控方案。

心脏包含免疫细胞的异构组，在心脏炎症和修复中起着至关重要的作用。为了了解免疫细胞的异质性，并进一步洞察其功能，重要的是要有一种方法，将允许这些细胞从心脏恢复。这种技术可以帮助发现存在于健康或患病心脏中的免疫和非免疫细胞多样性。

这种技术有三个主要优点。首先，它是一个简单的单一工作流程，导致在三小时内生成各种细胞类型的单细胞悬浮液。其次，它导致活的免疫和非免疫细胞群的高恢复。

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免疫学和感染第154期巨噬细胞人类心脏流细胞学心力衰竭基质细胞小鼠心脏