Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Discrimintion and Mapping of the Primary and…
JoVE Journal
Genetics

需要订阅 JoVE 才能查看此 内容.

JoVE Journal Genetics
Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
Production and Purification of Baculovirus for Gene Therapy Application
Journal (Genetics)
Production and Purification of Baculovirus for Gene Therapy Application
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
Journal (Genetics)
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
 

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Article DOI: 10.3791/60019-v • 07:26 min • July 29th, 2019
July 29th, 2019 •
1,2, 2, 2, 2, 2, 1,2, 1,2, 1,2
1State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, 2Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University

章节

总结概括
Automatic Translation

We present a circular RT-PCR-based strategy by combining circular RT-PCR, quantitative RT-PCR, RNA 5' polyphosphatase-treatment, and Northern blot. This protocol includes a normalization step to minimize the influence of unstable 5' triphosphate, and it is suitable for discriminating and mapping the primary and processed transcripts stably accumulated in maize mitochondrion.

Tags

Discrimination Mapping Primary Transcripts Processed Transcripts Maize Mitochondria Circular RT-PCR-based Strategy RNA Normalization Five Prime Triphosphate Plastids Plant Mitochondria Developing Kernels Extraction Buffer Filter Cloth Centrifuge Supernatant Wash Buffer Mitochondrial Pellet RNA Extraction Polyphosphatase Treatment
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter