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DOI: 10.3791/60162-v
Marwa Belhaj1, Balasubramaniam Annamalai2, Nathaniel Parsons2, Andrew Shuler1, Jay Potts1, Bärbel Rohrer2,3,4
1Department of Cell Biology,University of South Carolina, 2Department of Ophthalmology, Division of Research,Medical University of South Carolina, 3Department of Neuroscience, Division of Research,Medical University of South Carolina, 4Division of Research,Ralph H. Johnson VA Medical Center
Please note that some of the translations on this page are AI generated. Click here for the English version.
此处介绍的是一种协议,用于将藻酸盐作为聚合物在永生细胞的微封装中,用于长期向啮齿动物的眼睛提供生物制剂。
该技术是将药物、蛋白质或细胞等任何内容传递到组织或动物中特定位置的一种可靠方法。您可以跟踪交付并调整内容的发布,以符合您想要的实验。它可用于治疗从伤口愈合到眼部和心脏病的许多疾病。
这种方法的主要优点是能够使用藻酸盐微胶囊将治疗细胞送到目标位点,而不会引起任何免疫反应,并持续持续提供新鲜分泌的治疗药物更长时间。首先根据手稿方向培养视网膜上皮细胞。使用标准组织培养程序,当细胞达到70至80%的汇合时,通过细胞。
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