Journal
/
生物学
/
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
JoVE 杂志
生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones

DOI:
10.3791/60197-v

•

10:11 min

•

September 16, 2019

•

, , , ,
1Department of Biomedical Research,University of Veterinary Medicine Vienna

Chapters

  • 00:04Title
  • 01:00Animals and Freeze Embedding
  • 01:41Section Preparation
  • 03:26Rapid Staining Protocol
  • 04:57Laser Capture Microdissection
  • 06:27RNA Extraction
  • 07:43Results: Analysis of RNA Obtained from Fresh-Frozen Mouse Bones
  • 09:10Conclusion

Summary

自动翻译

自动翻译

A laser capture microdissection (LCM) protocol was developed to obtain sufficient quantity of high-quality RNA for gene expression analysis in bone cells. The current study focusses on mouse femur sections. However, the LCM protocol reported here can be used to study gene expression in cells of any hard tissue.

Tags

Laser Capture MicrodissectionRNA ExtractionMouse BoneCryosectionsGene Expression AnalysisOCT CompoundCryostat

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Laser Capture Microdissection of Mammalian Tissue

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

A Web Tool for Generating High Quality Machine-readable Biological Pathways

A Simple High Efficiency Protocol for Pancreatic Islet Isolation from Mice

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Read Article