Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

Jane Harmer; Nina Struve; Anke Br&#252;ning-Richardson

doi:10.3791/60273

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

通过高分辨率共聚焦显微镜对3D肿瘤球体入侵的影响表征

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09:17 min

September 16, 2019

DOI: 10.3791/60273-v

Jane Harmer¹, Nina Struve², Anke Brüning-Richardson¹

¹School of Applied Sciences,University of Huddersfield, ²Laboratory of Radiotherapy and Experimental Radiation Oncology, Humbertus Wald Tumorzentrum,University Cancer Center Hamburg, University Medical Center Hamburg-Eppendorf

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使用组蛋白脱乙酰雅酶（HDAC）抑制剂的三维（3D）入侵测定中，压伤抑制剂对胶质瘤癌细胞迁移的影响以高分辨率共聚焦显微镜为特征。

该协议证明，我们现在可以在3D癌症模型中详细确定哑压抑制剂的效果。主要优点是采用一种易于和一对一的方法，可以采用这种方法。该技术允许在3D环境中评估癌症研究中的哑压药物，允许在肿瘤环境相关环境中测试药物。

这种方法可用于癌症细胞毒性药物的测试，或者，例如，评估辐射对癌症增殖和细胞迁移的影响。用普通的96孔板练习介质去除步骤和胶原蛋白替代是有用的。此外，在共合显微镜上进行培训也是必不可少的。

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癌症研究问题 151 癌细胞迁移胶质瘤球形 3D入侵测定抑制剂共聚焦显微镜