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Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups
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神经科学
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JoVE 杂志 神经科学
Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

DOI:
10.3791/60291-v

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10:35 min

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January 12, 2020

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, , , ,
1Department of Anatomy, Physiology and Cell Biology,University of California Davis, 2University of Veterinary Medicine Hannover Foundation

Chapters

  • 00:04Title
  • 00:40Central Nervous System (CNS) Tissue Dissection
  • 02:35CNS Tissue Homogenization
  • 04:20Microvessel Purification
  • 05:57Microvessel Elution and Filtration
  • 07:44Results: Representative Microvessel Protein Expression
  • 09:57Conclusion

Summary

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自动翻译

The goal of this protocol is to isolate microvessels from multiple regions of the central nervous system of lissencephalic and gyrencephalic vertebrates.

Tags

Microvessel IsolationCentral Nervous SystemVertebratesDissectionMeningesChoroid PlexusHomogenization

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