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癌症研究
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Isolation and Characterization of Patient-derived Pancreatic Ductal Adenocarcinoma Organoid Models
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癌症研究
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JoVE 杂志 癌症研究
Isolation and Characterization of Patient-derived Pancreatic Ductal Adenocarcinoma Organoid Models

Isolation and Characterization of Patient-derived Pancreatic Ductal Adenocarcinoma Organoid Models

DOI:
10.3791/60364-v

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08:43 min

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January 14, 2020

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, ,
1Moores Cancer Center,University of California San Diego, 2Department of Surgery, Division of Surgical Oncology,University of California San Diego

Chapters

  • 00:04Title
  • 00:46Passaging of PDAC Organoids
  • 04:59Characterization of PDAC Organoids
  • 06:36Results: Isolation and Characterization of Patient-Derived Pancreatic Ductal Adenocarcinoma Organoid Models
  • 07:55Conclusion

Summary

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Patient-derived organoid cultures of pancreatic ductal adenocarcinoma are a rapidly established 3-dimensional model that represent epithelial tumor cell compartments with high fidelity, enabling translational research into this lethal malignancy. Here, we provide detailed methods to establish and propagate organoids as well as to perform relevant biological assays using these models.

Tags

Pancreatic Ductal AdenocarcinomaOrganoid ModelsPatient-derivedGenetic HeterogeneityPhenotypic HeterogeneityCytotoxic DrugsDrug ResistanceBasement Membrane ExtractBMECell Recovery SolutionTrypsinDNAse I

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