Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad

Lauren  B. Shunkwiler; Jill  D. Haag; Michael  N. Gould; Bart M. G. Smits

doi:10.3791/60401

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad

大鼠乳腺内皮细胞移植到间膜白脂肪垫

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10:41 min

March 4, 2020

DOI: 10.3791/60401-v

Lauren B. Shunkwiler¹, Jill D. Haag², Michael N. Gould², Bart M. G. Smits¹

¹Department of Pathology and Laboratory Medicine,Medical University of South Carolina, ²Department of Oncology, McArdle Laboratory for Cancer Research,University of Wisconsin-Madison School of Medicine and Public Health

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本文介绍了一种移植方法，将供体大鼠乳腺上皮细胞移植到受体动物的细胞间白脂肪垫中。该方法可用于检查宿主和/或供体对乳腺上皮发育的影响，并消除预清除的需要，从而扩展了此技术的实用性。

该协议已经使用了40多年，仍然是大鼠乳腺细胞移植检测的唯一可靠方法。该方法在乳腺癌易感性研究中被广泛用于研究乳腺细胞自主性与宿主效应。通过移植在细胞间脂肪垫中，这种方法避免了必须清除内源膜上皮，这在大鼠身上无效。

因此，该方法的侵入性较小，内源性乳腺可用作内部控制。要开始这个程序，把一只安乐死的雌性大鼠放在它的背上，用针钉固定它。用70%乙醇喷洒整个腹体表面。

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癌症研究问题 157 大鼠乳腺上皮移植单分散细胞定量乳腺发育乳腺癌癌移植