从产前 Islmn:GFP 转基因小鼠中分离与培养动眼神经、滑车神经及脊髓运动神经元
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被引用 5 次
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10:04 分钟
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2019年11月12日
DOI :10.3791/60440-v
2019年11月12日
•10.5K 次观看
Ryosuke Fujiki1,2,3,4,9 , Joun Y. Lee1,2,10 , Julie A. Jurgens1,2,3,7 , Mary C. Whitman2,5,6 , Elizabeth C. Engle1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine
本研究提出了一种用于获得均一的原代动眼神经、滑车神经及脊髓运动神经元细胞培养物的实验方案。这些细胞培养物可用于比较分析眼外肌运动神经元与脊髓运动神经元在形态学、细胞学、分子生物学以及电生理学特性方面的差异。
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Chapters in this video
0:05
Title
1:17
Ventral Midbrain and Spinal Cord Dissection
6:10
Tissue Dissociation
7:23
Culture of Purified Primary Motor Neurons
7:59
Results: Immunohistochemistry of CN3/CN4 and SMN Isolated Cultures
9:34
Conclusion
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