Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for <em>Acinetobacter baumannii</em>

Melina B. Cian; Nicole P. Giordano; Joshua A. Mettlach; Keaton  E. Minor; Zachary D. Dalebroux

doi:10.3791/60517

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

将葛兰阴性细菌的细胞包络分离到内膜和外膜分数，对阿辛托巴基的培养素进行技术调整

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10:24 min

April 10, 2020

DOI: 10.3791/60517-v

Melina B. Cian*¹, Nicole P. Giordano*¹, Joshua A. Mettlach*¹, Keaton E. Minor*¹, Zachary D. Dalebroux¹

¹Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center

Please note that some of the translations on this page are AI generated. Click here for the English version.

革兰氏阴性细菌产生两个空间隔离膜。外膜由围皮层和气膜层从内膜分割。分离这些微生物的双层的能力对于了解它们的生理和发病机制至关重要。

研究微生物如何调节单个双层的化学成分，将告知我们对抗生素杀灭、抗微生物药物耐药性和疾病发病机制的了解。此技术将克阴性细菌的细胞包络分割成两个定义的分数，而无需使用洗涤剂。因此，脂质、蛋白质和糖可以在半原生的环境中进行评估。

有些步骤取决于时间，需要重点和协调。最初，使用一个或两个示例。当一个人的舒适度升高时，可以添加更多的样品。

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