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Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss

Article DOI: 10.3791/60542-v • 06:23 min • April 24th, 2020
April 24th, 2020 •
*1, *2, 1, 1, 2, 2, 3, 3, 3, 1,2
1Laboratory for Angiogenesis and Ocular Cell Transplantation, University of Lübeck, 2Department of Ophthalmology, University of Lübeck, 3Institute of Biomedical Optics, University of Lübeck
* These authors contributed equally

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Here, we present a protocol to detach corneal endothelial cells (CEC) from Descemet’s membrane (DM) using a neodymium:YAG (Nd:YAG) laser as an ex vivo disease model for bullous keratopathy (BK).

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Noninvasive Laser-assisted Experimental Model Corneal Endothelial Cell Loss Disease Models Stem Cells Gene Therapy Early Stages Of The Disease Photodisruption Nd:YAG Laser Ophthalmologists Porcine Eyes Extracellular Tissues Povidone Iodine Ophthalmic Solution Sterile PBS Spectral Domain Optical Coherence Tomography Device Corneal Scarring Edema Opacities Slip Lamp Unit
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