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Bioengineering
中国仓鼠卵巢细胞培养中高通量自动化微生物反应器工艺优化
中国仓鼠卵巢细胞培养中高通量自动化微生物反应器工艺优化
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

中国仓鼠卵巢细胞培养中高通量自动化微生物反应器工艺优化

Full Text
9,259 Views
09:28 min
May 18, 2020

DOI: 10.3791/60577-v

Tamanna Nagraik1, Alina Gonzalez Salcedo1, Dörte Solle1, Thomas Scheper1

1Institute for Technical Chemistry,Gottfried-Wilhelm-Leibniz Universität Hannover

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Please note that some of the translations on this page are AI generated. Click here for the English version.

在这里,我们提出了一个详细的过程,在自动化的微生物反应器中运行实验设计,然后使用蛋白质A柱进行细胞收获和蛋白质定量。

在这段视频中,用户通过规划和执行实验或DOE的设计,引入到微生物反应器软件中。还演示了分析仪辅助阀,以优化工艺条件。将 modet 并入自动化微生物反应器软件已正式用于数据分析。

大量的实验可以小规模地同时进行规划和执行。演示这个程序的将是塔曼娜·纳格雷克,一个来自我实验室的博士生。从主要培养中的培养前程序开始,如文本协议中所述。

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