用骨肉瘤衍生细胞外囊泡处理的美相干细胞中的LINE-1甲基化分析
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February 01, 2020
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这里描述的是使用甲基化特异性探针扩增方法分析使用骨肉瘤衍生的细胞外囊泡治疗的中位体干细胞中LINE-1元素的甲基化水平。也演示了超中心化，一种从胎儿牛血清中分离细胞外囊泡的常用程序。
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Cite this Article
Sinha, S., Mannerström, B., Seppänen-Kaijansinkko, R., Kaur, S. LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles. J. Vis. Exp. (156), e60705, doi:10.3791/60705 (2020).
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