使用荧光抗生素探针对细菌耐药性的可视化
12,633 Views
•
08:23 min
•
March 02, 2020
DOI:
Summary
Automatically generated
荧光标记的抗生素是强大的工具，可用于研究抗菌素耐药性的多个方面。本文介绍了荧光标记抗生素的制备及其在细菌中抗生素耐药性研究中的应用。探针可用于通过分光光度测定法、流式细胞测定和显微镜研究细菌抗性机制（例如，排泄物）。
Related Videos
07:40
Monitoring Spatial Segregation in Surface Colonizing Microbial Populations
Related Videos
11005 Views
07:10
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Related Videos
8824 Views
12:41
Stress-induced Antibiotic Susceptibility Testing on a Chip
Related Videos
6394 Views
12:32
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
Related Videos
13922 Views
05:57
Live-Cell Fluorescence Microscopy to Investigate Subcellular Protein Localization and Cell Morphology Changes in Bacteria
Related Videos
7065 Views
08:33
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
Related Videos
6886 Views
06:45
Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Related Videos
8541 Views
07:28
Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
Related Videos
15783 Views
12:29
Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
Related Videos
1896 Views
07:44
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
Related Videos
794 Views
Read Article
Cite this Article
Stone, M. R. L., Phetsang, W., Cooper, M. A., Blaskovich, M. A. T. Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes. J. Vis. Exp. (157), e60743, doi:10.3791/60743 (2020).
Copy