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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Article DOI: 10.3791/60905-v • 07:29 min • May 16th, 2020
May 16th, 2020 •
*1, *1, 2, 1,3
1Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 2Department of Dermatology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands, 3Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands
* These authors contributed equally

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Presented here is a stepwise procedure for in vitro differentiation of human primary keratinocytes by contact inhibition followed by characterization at the molecular level by RNA-seq analysis.

Tags

In Vitro Differentiation Human Primary Keratinocytes RNA-Seq Analysis 2D Keratinocyte Differentiation Contact Inhibition Molecular Characterization Epidermal Differentiation Epigenomic Analysis RNA-seq Analysis Pipeline Seeding Density Keratinocyte Growth Medium Proliferation Medium Differentiation Medium Cell Collection
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