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JoVE Journal Medicine

Snijden en kweken Varkensharten onder fysiologische omstandigheden

Measurement of Carotenoids in Perifovea using the Macular Pigment Reflectometer

Journal (Medicine)

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Journal (Medicine)

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Snijden en kweken Varkensharten onder fysiologische omstandigheden

Article DOI: 10.3791/60913-v • 07:54 min • March 20th, 2020

March 20th, 2020 •

Qinghui Ou*¹, Riham R.E. Abouleisa*¹, Xian-Liang Tang¹, Hamzah R. Juhardeen¹, Moustafa H. Meki², Jessica M. Miller², Guruprasad Giridharan², Ayman El-Baz², Roberto Bolli¹, Tamer M.A. Mohamed^1,3,4,5,6

¹Institute of Molecular Cardiology, Department of Medicine, University of Louisville, ²Department of Bioengineering, University of Louisville, ³Diabetes and Obesity Center, Department of Medicine, University of Louisville, ⁴Department of Pharmacology and Toxicology, University of Louisville, ⁵Institute of Cardiovascular Sciences, University of Manchester, ⁶Faculty of Pharmacy, Zagazig University

* These authors contributed equally

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Dit protocol beschrijft hoe hartweefsel te snijden en te kweken onder fysiologische omstandigheden gedurende 6 dagen. Dit cultuursysteem kan worden gebruikt als een platform voor het testen van de werkzaamheid van nieuwe harthartfalen therapieën evenals betrouwbare testen van acute cardiotoxiciteit in een 3D-hartmodel.

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Geneeskunde Nummer 157 gentherapie cardiotoxiciteit hart elektrische stimulatie calcium elektrofysiologie biomimetische cultuur farmacologie

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