Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

David C. Montrose; Erin M. McNally; Erika Sue; Andrew J. Dannenberg

doi:10.3791/60949

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

在小鼠中进行结肠镜引导捏活检并评估后续组织变化

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08:52 min

February 5, 2021

DOI: 10.3791/60949-v

David C. Montrose^1,2, Erin M. McNally³, Erika Sue³, Andrew J. Dannenberg³

¹Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, ²Stony Brook Cancer Center, Stony Brook, NY, ³Department of Medicine,Weill Cornell Medicine, New York, NY

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在这里，我们提供了一个详细的程序，诱导小鼠结肠镜引导捏活检和跟踪伤口关闭的实时程序。此外，还提供了组织准备方法，用于对伤口床进行组织学、免疫组织化学和分子分析。

该方法为研究结肠伤口愈合以及损伤后发生的分子和组织学变化提供了理想的方法。这种方法还可以揭示IBD发病机制。该技术的主要优点是精确控制损伤的位置和伤口愈合过程的时间。

首次执行此方法的个人可能难以将结肠镜插入鼠标的结肠并产生一致的伤口。反复练习这些技术应该能克服这些问题。鉴于此过程涉及多个协调步骤和细微差别，此方法的视觉演示至关重要。

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免疫学和感染第168期活检伤口愈合结肠小鼠结肠镜检查损伤