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Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro

Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro

DOI:
10.3791/60952-v

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08:16 min

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May 20, 2020

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, , , ,
1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair,Nantong University

Chapters

  • 00:04Introduction
  • 00:45Nerve Dissection and Cell Culture
  • 02:32Isolation and culture of Schwann Cells (SCs)
  • 03:43Isolation and Culture of Fibroblasts
  • 05:27Results: Morphology and Purity of Schwann Cells and Fibroblasts
  • 07:48Conclusion

Summary

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Here, we present a method to purify fibroblasts and Schwann cells from sensory and motor nerves in vitro.

Tags

FibroblastsSchwann CellsNerve RegenerationNervous System DiseasesVentral RootDorsal RootTrypsinDMEMFBSPBSCell CultureCell Purification

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