用于大型精子细胞三维共焦分析的全山 嗜血杆菌 睾丸免疫染色
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08:37 min
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August 27, 2020
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我们在这里提出了一个改进的协议，为全安装准备和免疫染色的 嗜血杆菌 睾丸，适合共焦显微镜，也允许可重复和可靠的标签。我们通过对大型S4-S5精子细胞的同位素化实验进行3D表示和量化来说明此协议。
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Cite this Article
Raich, N., Contremoulins, V., Karess, R. E. Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes. J. Vis. Exp. (162), e61061, doi:10.3791/61061 (2020).
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