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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifisering og karakterisering av immunogene RNA-arter i HDM-allergener som modulerer eosinofil lungebetennelse
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Identifisering og karakterisering av immunogene RNA-arter i HDM-allergener som modulerer eosinofil lungebetennelse

Article DOI: 10.3791/61183-v • 08:44 min • May 30th, 2020
May 30th, 2020 •
1,2, 1,3, 1
1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 3Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

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Miljøallergener som husstøvmidd (HDM) inneholder ofte mikrobielle stoffer som aktiverer medfødte immunresponser for å regulere allergisk betennelse. Protokollen som presenteres her viser identifisering av dsRNA arter i HDM allergener og karakterisering av deres immunogene aktiviteter i modulerende eosinofillungebetennelse.

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Immunologi og infeksjon utgave 159 BAL bronkoalveoler lavage dsRNA FACS fluorescensaktivert cellesortering HDM husstøvmidd ISGs interferon stimulerte gener PAMP patogen-assosiert molekylært mønster RT-qPCR
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