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Journal / Genetics / 小鼠肾中微RNA表达与单体尿阻的定量实时PCR评价…
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JoVE Journal Genetics
小鼠肾中微RNA表达与单体尿阻的定量实时PCR评价
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小鼠肾中微RNA表达与单体尿阻的定量实时PCR评价

Article DOI: 10.3791/61383-v • 07:36 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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我们描述了一种通过定量逆转录聚合酶链反应评估具有单性尿道阻塞（UUO）小鼠肾脏微RNA表达的方法。该协议适用于研究小鼠的肾微RNA表达配置文件与UOU和其他病理条件的背景下。

Tags

遗传学， 问题 162， 分子生物学， 微RNA， 补充DNA， 单体尿道阻塞， 肾脏， 肾脏间质纤维化， qRT-PCR
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