条件性Stat3基因敲除小鼠模型的骨骼表型分析
4.1K 次观看
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被引用 4 次
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08:42 分钟
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2020年7月3日
DOI :10.3791/61390-v
2020年7月3日
•4.1K 次观看
Yiling Yang*1 , Qianye Chen*2 , Siru Zhou1 , Xinyi Gong1 , Hongyuan Xu1 , Yueyang Hong1 , Qinggang Dai3 , Lingyong Jiang1
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, 2Department of Stomatology, Dalian Medical University, 3The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology
* These authors contributed equally
本方案描述了一种用于理解体内调控破骨细胞活性的关键基因的经典方法。该方法利用转基因小鼠模型和一些经典技术来分析骨骼表型。
Chapters in this video
0:04
Introduction
0:54
Specimen Collection
2:13
Paraffin Section Preparation
3:34
TRAP Staining
5:34
Calcein and Alizarin Red Double Labeling
6:55
Results: Effects of Stat3 Deletion on Osteoclast Differentiation
8:09
Conclusion
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