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In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation

Article DOI: 10.3791/61439-v • 11:07 min • May 11th, 2020
May 11th, 2020 •
*1, *1
1Department of Neurobiology, Poznań University of Physical Education
* These authors contributed equally

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This protocol describes in vivo intracellular recording of rat lumbar motoneurons with simultaneous trans-spinal direct current stimulation. The method enables us to measure membrane properties and to record rhythmic firing of motoneurons before, during and after anodal or cathodal polarization of the spinal cord.

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Intracellular Recording Spinal Motoneurons Trans-spinal Direct Current Stimulation Nervous System Practical Applications Anesthetized Rat Dissecting Microscope Skin Incision Jugular Vein Ligatures Catheter Placement Tracheal Tube Placement
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