An Ex vivo Assay to Study <em>Candida albicans</em> Hyphal Morphogenesis in the Gastrointestinal Tract

Ross Monasky; Sonia Villa; Shankar Thangamani

doi:10.3791/61488

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

前体内分析研究胃肠道的念珠菌血脂磷

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07:42 min

July 1, 2020

DOI: 10.3791/61488-v

Ross Monasky¹, Sonia Villa², Shankar Thangamani³

¹College of Veterinary Medicine,Midwestern University, ²Masters in Biomedical Science Program, College of Graduate Studies,Midwestern University, ³Department of Pathology and Population Medicine, College of Veterinary Medicine,Midwestern University

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本研究中描述的利用肠道同质化提取物和免疫荧光染色的外体检测是一种研究胃肠道中念珠菌的催眠形态的新方法。该方法可用于研究调节肠道形态遗传过渡的环境信号。

本视频中演示的协议使我们能够了解肠道代谢物在真菌催眠形态生成中的作用。这种外体技术或多或少是肠道研究真菌催眠形态的最接近近似值，无法进行体外研究。该方法可用于研究肠道代谢物在其他肠道病原体中的作用。

使用解洗消毒的尖端剪刀和钳子解剖小鼠。安乐死后，通过固定所有四肢露出腹部，将动物固定在解剖表面。用70%乙醇喷洒腹部区域，防止毛皮粘在钳子、剪刀或肠道部分。

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免疫学和感染问题 161 念珠菌催眠病原体外分析葡萄糖继发性胆汁酸和胃肠道