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Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
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Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

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In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation models.

Tags

Quantification Circulating Pig-specific DNA Blood Xenotransplantation Model Immune Rejection Monitoring Protocol Simple Method Convenient Method Low Cost Method Less Invasive Method Pig-to-human Islet Transplantation Kidney Transplantation Liver Transplantation Heart Transplantation Blood Samples Micro Centrifuge Tubes Protease K Lysis Buffer Water Bath Incubation Clear Solution Liquid Beads Anhydrous Ethyl Alcohol Adsorption Column Centrifuge Rinse Solution
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