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Bioengineering

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Manipolazione di singole cellule staminali neurali e neuroni nelle fette cerebrali utilizzando la microiniezione robotica

Article DOI: 10.3791/61599-v • 11:40 min • January 21st, 2021
January 21st, 2021 •
*1,2, *3, 3, 3,4, 1,5,6
1Department of Biomedical Engineering, University of Minnesota, 2Department of Biomedical Engineering, Duke University, 3Max Planck Institute of Molecular Cell Biology and Genetics, 4Max Planck Institute for Evolutionary Anthropology, 5Department of Mechanical Engineering, University of Minnesota, 6Graduate Program in Neuroscience, University of Minnesota
* These authors contributed equally

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Questo protocollo dimostra l'uso di una piattaforma robotica per la microiniezione in singole cellule staminali neurali e neuroni nelle fette cerebrali. Questa tecnica è versatile e offre un metodo per tracciare le cellule nei tessuti ad alta risoluzione spaziale.

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Bioingegneria Numero 167 sviluppo cerebrale cellule staminali tracciamento del lignaggio robotica neurone microiniezione fetta di tessuto organotipico manipolazione di singole cellule
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