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Immunology and Infection

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JoVE Journal Immunology and Infection

Kistik Fibrozis Hastalarının Balgamında Pseudomonas aeruginosa'nın Görüntülenmesi

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Journal (Immunology and Infection)

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

Journal (Immunology and Infection)

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci

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Kistik Fibrozis Hastalarının Balgamında Pseudomonas aeruginosa'nın Görüntülenmesi

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020

July 16th, 2020 •

Lindsay Jackson¹, William DePas², Amanda J. Morris¹, Kevin Guttman¹, Yvonne C W Yau^1,3, Valerie Waters^1,4

¹Translational Medicine, Hospital for Sick Children, ²Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, ³Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, ⁴Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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Bu protokol, kistik fibrozis hastalarının balgamındaki bakteri hücrelerinin ve polisakkarit sentez lokusu (Psl) polisakkaritinin görüntülenmesi için yöntemler sağlar.

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İmmünoloji ve Enfeksiyon Sayı 161 Kronik Enfeksiyon Akciğer Fonksiyonu Morbidite Antibiyotik Tedavisi Çocuk Hastalar Mekansal Organizasyon Agregasyon Biyofilm Oluşumu Polisakkarit Üretimi Balgam Örnekleri Doku Temizleme Tekniği Hidrojel Matriks 3 Boyutlu Yapılar Floresan Etiketler Floresan In Situ Hibridizasyon Anti-Psl-antikorlar Ekzopolisakkarit DAPI Boyama Konak Hücreler Yüksek Çözünürlüklü Görüntüleme

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