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Bioengineering

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Building a Simple and Versatile Illumination System for Optogenetic Experiments

Article DOI: 10.3791/61914-v • 06:41 min • January 12th, 2021
January 12th, 2021 •
1, 2, 1, 1, 1, 3, 1, 1, 4, 1, 1
1Department of Bioengineering, University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology, University of California, Irvine, 4Department of Biomedical Engineering, Duke University

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This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.

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Illumination System Optogenetic Experiments Inexpensive Versatile LED Illumination Timing Wavelengths Visible Near IR Spectrum Electrical Circuit Printed Circuit Board Jumper Wires Soldering Flux Resistor Arduino Connector LEDs Tinning Wire Soldering Tip
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