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Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Article DOI: 10.3791/61962-v • 10:41 min • January 23rd, 2021
January 23rd, 2021 •
1,2,3,4, 2,3,4
1University of Newcastle, 2University of Sydney, 3Kolling Institute of Medical Research, Royal North Shore Hospital, 4University of Technology, Sydney

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This protocol aims to fabricate 3D cardiac spheroids (CSs) by co-culturing cells in hanging drops. Collagen-embedded CSs are treated with doxorubicin (DOX, a cardiotoxic agent) at physiological concentrations to model heart failure. In vitro testing using DOX-treated CSs may be used to identify novel therapies for heart failure patients.

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Cardiac Spheroids In Vitro Bioengineered Heart Tissues Human Heart Pathophysiology Vascularization Co-culturing Cells Cardiac Myocytes Cardiac Fibroblasts Cardiac Endothelial Cells Human Heart Microenvironment Drug Toxicity Assays Doxorubicin Cardiotoxic Drug Cryo Vial Plating Medium
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