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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Article DOI: 10.3791/62016-v • 11:42 min • June 10th, 2021
June 10th, 2021 •
1, 1, 1, 1, 1, 1, 1,2
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

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We describe a protocol to reprogram human skin-derived fibroblasts into induced Neuronal Progenitor Cells (iNPCs), and their subsequent differentiation into induced Astrocytes (iAs). This method leads to fast and reproducible generation of iNPCs and iAs in large quantities.

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In Vitro Modeling Neurological Diseases Direct Conversion Fibroblasts Neuronal Progenitor Cells Astrocytes IPS Technology Disease Phenotype Skin Samples Role Of Astrocytes Medications Disease Mechanisms Co-cultures Primary Human Skin Fibroblasts Cell Banks Skin Biopsies Tissue Culture Incubator Fibronectin
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