生物组织样本的串行块面扫描电子显微镜 （SBF-SEM）
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March 26, 2021
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Summary
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该协议概述了使用串行块面扫描电子显微镜 （SBF-SEM） 的常规方法，这是一种强大的 3D 成像技术。SBF-SEM 的成功应用取决于适当的固定和组织染色技术，以及仔细考虑成像设置。本协议包含整个过程的实际考虑。
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Cite this Article
Courson, J. A., Landry, P. T., Do, T., Spehlmann, E., Lafontant, P. J., Patel, N., Rumbaut, R. E., Burns, A. R. Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples. J. Vis. Exp. (169), e62045, doi:10.3791/62045 (2021).
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