无接触共培养模型用于研究呼吸道病毒感染期间的固有免疫细胞活化
2.8K 次观看
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被引用 1 次
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07:36 分钟
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2021年2月28日
DOI :10.3791/62115-v
2021年2月28日
•2.8K 次观看
Zhe Zhang Ryan Lew*1 , Jing Liu*1 , Hsiao Hui Ong1 , Vivian Jiayi Tan2 , Annika Luukkainen3 , Yew Kwang Ong1,4 , Mark Thong1,4 , Kia Joo Puan5 , Vincent Tak Kwong Chow2,6 , Kai Sen Tan1,2,6 , De Yun Wang1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
* These authors contributed equally
本方案详细描述了病毒感染的鼻上皮细胞与天然免疫细胞活化之间早期相互作用的研究方法。可根据免疫细胞亚群在病毒感染后的活化状态对其进行区分，并进一步研究其对早期抗病毒反应的影响。
Chapters in this video
0:04
Introduction
0:50
hNEC Viral Infection and hNEC + PBMCs Co-Culture
3:30
Harvesting of PBMCs 48/72 h Post-Viral Infection
5:31
Results: Activation of T Cells Following Influenza Infection
6:56
Conclusion
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