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Chapters
- 00:05Introduction
- 00:43Slam-Freezing Equipment Preparation
- 01:26Sample Collection and Slam-Freezing
- 02:22Rhizosphere Cube Freeze-Drying and Embedding
- 03:53Rhizosphere Cubes Trimming and Sectioning
- 04:18Results: Elemental Wetland Root and Rhizosphere Preservation and Imaging
- 05:53Conclusion
We describe a protocol to sample, preserve, and section intact roots and the surrounding rhizosphere soil from wetland environments using rice (Oryza sativa L.) as a model species. Once preserved, the sample can be analyzed using elemental imaging techniques, such as synchrotron X-ray fluorescence (XRF) chemical speciation imaging.
