评估具有FRET敏化发射的活细胞中的蛋白质相互作用
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April 22, 2021
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Summary
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两个荧光团分子之间的福斯特共振能量转移（FRET）可用于研究活细胞中的蛋白质相互作用。在这里，提供了有关如何通过使用共聚焦激光扫描显微镜检测受体的敏化发射和供体分子的淬灭来测量活细胞中的FRET的协议。
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Cite this Article
Vámosi, G., Miller, S., Sinha, M., Fernandez, M. K., Mocsár, G., Renz, M. Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission. J. Vis. Exp. (170), e62241, doi:10.3791/62241 (2021).
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