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小鼠肝细胞类器官的建立与遗传操作

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

JoVE 杂志
生物学
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JoVE 杂志生物学

Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

小鼠肝细胞类器官的建立与遗传操作

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4,762 Views

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14:54 min

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February 12, 2022

DOI:

10.3791/62438-v

DOI:

10.3791/62438-v

•

14:54 min

February 12, 2022

•

4709 Views

Jiabei Lian*^1,2, Xinyuan Meng*^1,3, Xiaohui Zhang^1,2, Huili Hu^1,2

¹Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine,Shandong University ²The Research Center of Stem Cell and Regenerative Medicine,Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University

Summary

从小鼠肝细胞建立了长期 的体外 3D类器官培养系统。这些类器官可以通过shRNA/异位构建的慢病毒感染，siRNA转染和CRISPR-Cas9工程进行遗传和遗传操纵。

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