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JoVE Journal
Immunology and Infection
使用共聚焦显微镜研究 利什曼原虫 的吞噬作用
使用共聚焦显微镜研究 利什曼原虫 的吞噬作用
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Investigating the Phagocytosis of Leishmania using Confocal Microscopy

使用共聚焦显微镜研究 利什曼原虫 的吞噬作用

Full Text
4,496 Views
08:41 min
July 29, 2021

DOI: 10.3791/62459-v

Amanda R. Paixão*1, Beatriz R. S. Dias*1, Luana C. Palma1, Natália M. Tavares1, Cláudia I. Brodskyn1, Juliana P. B de Menezes1, Patricia S. T. Veras1,2

1Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, 2National Institute of Science and Technology of Tropical Diseases - National Council for Scientific Research and Development (CNPq)

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Please note that some of the translations on this page are AI generated. Click here for the English version.

利 什曼原虫 感染中与吞噬作用相关的机制仍然知之甚少。在这里,我们描述了评估 利什曼原虫 与宿主细胞相互作用期间发生的早期事件的方法。

利什曼原虫感染中吞噬作用的相关机制仍然知之甚少。在这里,我们描述了评估利什曼原虫与宿主细胞相互作用期间早期固化状态的方法。该技术作为主要优点,允许研究利什曼原虫吞噬作用的不同状态和几种蛋白质的参与。

值得注意的是,这种技术也可以扩展到其他类型的研究,包括细菌、酵母菌感染,或者被其他类型的细胞吞噬。尝试这种技术的人应该注意细胞暴露于核苷酸溶液的时间。此外,我们建议开始测试转染,最多使用两个质粒。

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