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Biochemistry

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JoVE Journal Biochemistry
Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
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Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Article DOI: 10.3791/62470-v • 08:17 min • July 9th, 2021
July 9th, 2021 •
1, 1, 1
1Department of Medical Biochemistry and Biophysics, Karolinska Institutet

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We present a protocol to measure micro- to millisecond dynamics on 13C/15N-labeled and unlabeled RNA with 1H R1ρ relaxation dispersion nuclear magnetic resonance (NMR) spectroscopy. The focus of this protocol lies in high-purity sample preparation and setup of NMR experiments.

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RNA Sample Preparation NMR Setup Proton R1rho Relaxation Dispersion Confirmational Exchange Shifted Base-pairing Isotopic Labeling In Vitro Transcription Cleavage Reaction Denaturing PAGE Gel Incomplete Cleavage Microwave Heating Reanneal RNA Inorganic Pyrophosphatase RNase H
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