三维 体外 基于胶原支架的神经母细胞瘤仿生模型
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被引用 9 次
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07:48 分钟
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2021年7月9日
DOI :10.3791/62627-v
2021年7月9日
•3.8K 次观看
Ciara Gallagher*1,2,3 , Catherine Murphy*1,2,3 , Graeme Kelly4 , Fergal J. O’Brien3,5,6 , Olga Piskareva1,2,3,6,7
1Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 3Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 4National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland, 5Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, 6Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland
* These authors contributed equally
本文列出了在先前描述的三维胶原基支架上接种神经母细胞瘤细胞系所需的步骤，包括在预定时间内维持细胞生长，并回收支架以进行多种细胞生长和细胞行为分析及后续应用，可根据不同的实验目的进行调整。
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Chapters in this video
0:04
Introduction
0:45
Neuroblastoma Cell Culture on Scaffolds
2:52
Cell Viability Assessment Within Scaffolds
5:21
Results: Cell Viability and Morphology Assessment of Cells Grown on Scaffolds
7:10
Conclusion
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