Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

Journal / Biology /

JoVE Journal
Biology

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JoVE Journal Biology

Article DOI: 10.3791/62675-v • 09:31 min • July 22nd, 2021

July 22nd, 2021 •

This article describes the procedures used to evaluate the toxicity of UV radiation and chemical toxins on a primary and immortalized cell line.

Toxicity UV Radiation Chemicals Primary Human Corneal Epithelial Cells Immortalized Human Corneal Epithelial Cells In Vitro Ophthalmic Product Formulations Animal Testing Toxicity Endpoints Mitochondrial Function Cell Membrane Integrity Enzyme Activity Maximum Tolerable Dose Therapeutic Chemicals Eye Nijani Nagaarudkumaran Jones Laboratory PHCECs IHCECs Collagen Coated Petri Dishes Glass Bottom Cover Slips Human Ocular Epithelium Medium (HOEM) Incubator Carbon Dioxide Annexin Staining Buffer Solution Calcein Ethidium Homodimer 1 Annexin 5 Confocal Laser Scanning Microscope