通过肿瘤发生中定义基因功能 离体 恶性周围神经鞘瘤细胞中 floxed 等位基因的敲除
此处，我们介绍一种针对胚胎致死基因敲除的实验方案 体内 在基因工程小鼠模型来源的肿瘤中进行基因敲除，随后评估该敲除对肿瘤生长、增殖、存活、迁移、侵袭以及转录组的影响 体外 和 体内.
Chapters in this video
0:05
Introduction
0:52
Tumor Dissection and Staining
1:46
Ex Vivo Ablation of Floxed Erbb4 Alleles in MPNST Cells
3:52
Proliferation Assays in MPNST Cells with Ablated Erbb4 Alleles
4:23
RNA-Seq Analyses and Gene Identification with Altered Expression by Erbb4 Loss
6:04
Orthotopic Allograft of MPNST Cells with Ablated Erbb4 Alleles and Erbb4 Ablation Effects Analysis
7:10
Results: Effect of Adenovirus Transduction on MPNST Cells and Allografts
9:09
Conclusion
Related Videos
版权所有 © 2026 MyJoVE Corporation。保留所有权利。