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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Article DOI: 10.3791/62765-v • 09:16 min • February 7th, 2022
February 7th, 2022 •
*1, *1, *1, 1
1Department of Chemistry and Biochemistry, University of California, San Diego
* These authors contributed equally

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This manuscript outlines the blot-and-plunge method to manually freeze biological specimens for single-particle cryogenic electron microscopy.

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Manual Plunging Biological Specimens Cryogenic Electron Microscopy Transmission Electron Microscopy Manual Plunger Cryogen Dewar Ethane Vessel Spinning Grid Storage Platform Manual Plunging Height Clamping Tweezers
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