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Journal / Bioengineering / 构建含有G蛋白偶联受体（GPCR）的脂质膜模型…
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JoVE Journal Bioengineering
构建含有G蛋白偶联受体（GPCR）的脂质膜模型
Procedure for Lung Engineering
Journal (Bioengineering)
Procedure for Lung Engineering
An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
Journal (Bioengineering)
An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
 
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构建含有G蛋白偶联受体（GPCR）的脂质膜模型

Article DOI: 10.3791/62830-v • 09:45 min • February 5th, 2022
February 5th, 2022 •
*1, *1, 1
1Mork Family Department of Chemical Engineering and Materials Science, University of Southern California
* These authors contributed equally

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该协议利用琼脂糖肿胀作为一种强大且可推广的技术，用于将整体膜蛋白（IMP）掺入巨型单层脂质囊泡（GUV）中，如本文所述，用于重建人1A 5-羟色胺受体蛋白（5-HT1AR），这是药理学上重要的G蛋白偶联受体之一。

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生物工程，第180期，
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