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Biochemistry

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Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
 

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Article DOI: 10.3791/62842-v 07:17 min August 2nd, 2021
August 2nd, 2021

章节

总结概括

Here, an experimental workflow is presented that enables the detection of caspase-8 processing directly at the death-inducing signaling complex (DISC) and determines the composition of this complex. This methodology has broad applications, from unraveling the molecular mechanisms of cell death pathways to the dynamic modeling of apoptosis networks.

Tags

CD95 Death-inducing Signaling Complex Procaspase-8 Apoptosis Initiation Caspases DISC Complex Caspase-8 Processing Cell Dish Medium CD95L Cell Suspension PBS Centrifuge
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