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Live-imaging of Mitochondrial System in Cultured Astrocytes
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Live-imaging of Mitochondrial System in Cultured Astrocytes

Live-imaging of Mitochondrial System in Cultured Astrocytes

DOI:
10.3791/62957-v

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06:20 min

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November 16, 2021

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, , ,
1Department of Psychiatry, Center for Psychiatric Neurosciences,Lausanne University Hospital (CHUV) and University of Lausanne

Chapters

  • 00:04Introduction
  • 00:44Rat Hippocampal Astrocyte Primary Culture
  • 01:25Long-Term Monitoring of the Mitochondrial System
  • 02:13Analysis of Individual Mitochondrial Morphology and LV-G1-MitoTimer Ratio
  • 03:54Analysis of Mitochondrial Motility
  • 04:28Data Transformation, Normalization, and Statistical Analysis
  • 04:51Results: Analysis of the Effects of H2O2 on the Mitochondrial System of Astrocytes
  • 05:53Conclusion

Summary

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This article describes the method for mitochondrial time-lapse imaging of astrocyte cultures equipped with MitoTimer biosensor and the resulting multiparametric analysis of mitochondrial dynamics, mobility, morphology, biogenesis, redox state, and turnover.

Tags

Live-imagingMitochondrial SystemCultured AstrocytesMicroscopyMitochondrial DynamicsLentiviral VectorMitoTimerFluorescence ImagingImage ProcessingSegmentationMitochondrial Measurements

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