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Generering af transgenik og knockouts i Strongyloides Species ved mikroinjektion

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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Det funktionelle genomiske værktøjssæt til de parasitære nematoder Strongyloides stercoralis og Strongyloides ratti omfatter transgenese, CRISPR/Cas9-medieret mutagenese og RNAi. Denne protokol vil demonstrere, hvordan man bruger intragonadal mikroinjektion til at introducere transgener og CRISPR-komponenter i S. stercoralis og S. ratti.

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Immunologi og infektion udgave 176
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