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Immunology and Infection

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JoVE Journal Immunology and Infection
Generere transgener og knockouts i Strongyloides arter ved mikroinjeksjon
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Generere transgener og knockouts i Strongyloides arter ved mikroinjeksjon

Article DOI: 10.3791/63023-v • 09:42 min • October 7th, 2021
October 7th, 2021 •
1, 1,2
1Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 2Molecular Biology Institute, University of California, Los Angeles

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Det funksjonelle genomiske verktøysettet for de parasittiske nematodene Strongyloides stercoralis og Strongyloides ratti inkluderer transgenese, CRISPR/Cas9-mediert mutagenese og RNAi. Denne protokollen vil demonstrere hvordan man bruker intragonadal mikroinjeksjon for å introdusere transgenes og CRISPR-komponenter i S. stercoralis og S. ratti.

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Immunologi og infeksjon utgave 176
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