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Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
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Biochemistry
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JoVE Journal Biochemistry
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

DOI:
10.3791/63046-v

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07:59 min

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August 19, 2021

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1Department of Biochemistry & Nutrition,Des Moines University

Chapters

  • 00:05Introduction
  • 00:27Determination of Glycogen Synthase Activity
  • 02:28Measurement of Released Glucose
  • 03:55Determination of Glycogen Branching
  • 05:34Results: Spectrophotometric Assays of Glycogen Synthase
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

Techniques to measure the activity of key enzymes of glycogen metabolism are presented, using a simple spectrophotometer operating in the visible range.

Tags

Keywords: Glycogen MetabolismSpectrophotometric MethodsGlycogen Synthase ActivityUDP GlucoseNADHGlycogenGlucose-6-phosphate DehydrogenaseHexokinaseEukaryotic Metabolism

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