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Establishment of a Mouse Severe Acute Pancreatitis Model using Retrograde Injection of Sodium Taurocholate into the Biliopancreatic Duct

Article DOI: 10.3791/63129-v • 07:10 min • April 1st, 2022
April 1st, 2022 •
1, 1, 1, 2,4,5, 1, 2,3,4,6
1Department of Gastroenterology, the First Affiliated Hospital of Soochow University, 2MOE Engineering Center of Hematological Disease, Soochow University, 3Cyrus Tang Hematology Center, Soochow University, 4National Clinical Research Center for Hematologic Diseases, the First Affiliated Hospital of Soochow University, 5MOH Key Lab of Thrombosis and Hemostasis, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 6Collaborative Innovation Center of Hematology, Soochow University

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A mouse model of severe acute pancreatitis is described herein. The procedure presented here is very rapid, simple, and accessible, thereby potentially allowing the study of the molecular mechanisms and different therapeutic interventions in acute pancreatitis in a convenient way.

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Mouse Severe Acute Pancreatitis Model Retrograde Injection Sodium Taurocholate Biliopancreatic Duct Animal Ethics Committee Surgical Procedures Anesthesia Fast The Mouse Sodium Taurocholate Solution Surgical Instruments Forceps Scissors Blade Holder Needle Holder Hemostatic Clip Vascular Clamps Sterile Drapes Micro-syringe Sterilization General Anesthesia Pentobarbital Sodium Solution Supine Position Depilation Cream
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